Used extensively as a fluorescent dye in biological imaging and microscopy, it is particularly valuable for labeling cell membranes due to its lipophilic nature. This dye is often employed in studies involving lipid bilayers, membrane dynamics, and cellular trafficking. Its bright fluorescence and photostability make it a reliable tool for tracking and visualizing live cells in real-time. Additionally, it is utilized in flow cytometry to analyze and sort cells based on membrane characteristics. The dye is also applied in neuroscience research to study neuronal membranes and synaptic activity. Its ability to integrate into lipid structures without disrupting their function makes it a preferred choice for various biophysical and biochemical applications.