DiI triflate is widely used in biological research as a fluorescent dye for cell labeling and tracking. It is particularly effective in staining cell membranes, allowing scientists to visualize and study cell dynamics, migration, and interactions. DiI triflate is commonly applied in neuroscience to trace neuronal pathways and map neural circuits due to its ability to diffuse along cell membranes. It is also used in developmental biology to monitor cell lineage and tissue formation. The dye's stability and bright fluorescence make it a valuable tool in live-cell imaging and long-term studies. Additionally, DiI triflate is utilized in flow cytometry and fluorescence microscopy to analyze cell populations and structures. Its compatibility with various cell types and minimal cytotoxicity further enhance its utility in experimental research.