Used primarily in biomedical imaging, this compound serves as a fluorescent probe for real-time visualization of biological processes. Its strong near-infrared fluorescence allows deep tissue penetration with minimal background interference, making it ideal for in vivo imaging. It is commonly employed in lymph node mapping, tumor detection, and vascular imaging during surgical procedures. The carboxylic acid functional group enables easy conjugation to targeting molecules such as antibodies, peptides, or nanoparticles, enhancing specificity toward diseased tissues. Additionally, it is utilized in diagnostic assays and drug delivery systems where optical tracking is required. Its compatibility with existing imaging platforms supports clinical translation and ongoing research in precision medicine.