Used as a fluorescent dye in biological staining and imaging, particularly for visualizing cell membranes and tracking membrane dynamics. Its lipophilic nature allows it to integrate into lipid bilayers, making it suitable for labeling live cells and monitoring cellular processes such as fusion, division, and membrane potential changes. Commonly employed in fluorescence microscopy and flow cytometry due to its fluorescence properties, which help reduce background autofluorescence and enhance image clarity. Also utilized in studies involving mitochondrial membrane potential and neuronal tracing because of its ability to accumulate in polarized mitochondria and axonal pathways.