Used as a fluorescent probe in biological imaging due to its strong near-infrared emission and high photostability. It selectively accumulates in mitochondria of living cells, making it valuable for tracking mitochondrial dynamics and membrane potential changes. Also applied in super-resolution microscopy techniques like STED and PALM, where its brightness and resistance to photobleaching improve image clarity. Additionally, it serves in sensing microenvironment polarity and viscosity in cellular compartments.