Used as a fluorescent probe in biological imaging due to its strong near-infrared emission and high photostability. It selectively accumulates in mitochondria of live cells, making it valuable for tracking mitochondrial dynamics and membrane potential changes. Its carboxyl groups allow for covalent conjugation to biomolecules such as antibodies or peptides, enabling targeted imaging in complex biological systems. Also employed in super-resolution microscopy techniques where long-term fluorescence tracking is required. Soluble in polar organic solvents and aqueous buffers when activated, it is suitable for both in vitro and cellular applications.