Used in fluorescence-based detection methods, particularly in immunohistochemistry and immunocytochemistry, to visualize specific antigens in tissues and cells. It enables highly sensitive signal amplification through the enzyme horseradish peroxidase (HRP), which catalyzes the deposition of the dye in close proximity to the target site. This allows for precise localization of proteins and nucleic acids with low background fluorescence. Commonly applied in multiplex staining when combined with other fluorophores, facilitating the simultaneous detection of multiple targets in a single sample. Also utilized in tyramide signal amplification (TSA) assays for enhanced detection of low-abundance biomarkers in research and diagnostic settings.