SLB Broth is commonly used in microbiological laboratories for the cultivation of fastidious microorganisms, particularly spirochetes such as Leptospira species. It supports the growth of these bacteria by providing essential nutrients, vitamins, and serum components in a liquid medium. The broth is especially valuable in diagnostic settings for isolating Leptospira from clinical samples like blood, urine, or cerebrospinal fluid during the early stages of leptospirosis. It is also used in research for maintaining and propagating cultures prior to identification or antigen preparation. Due to its enriched composition, SLB Broth helps sustain slow-growing organisms over extended incubation periods, making it suitable for both primary isolation and subculturing.