Sporulation Broth is primarily used in microbiology for inducing spore formation in bacteria, especially in species like Bacillus and Clostridium. It provides a nutrient-limited environment that triggers the cellular stress response necessary for sporulation. This broth is essential in laboratory settings where high numbers of bacterial spores are required for research, sterilization validation, and spore-based product development.

It is commonly used in studies related to spore germination, resistance mechanisms, and dormancy. The broth supports the complete lifecycle of spore-forming bacteria, making it valuable in environmental testing, pharmaceutical manufacturing (e.g., biological indicator preparation), and vaccine development. Due to its consistent formulation, it ensures reproducible sporulation across experiments, which is critical for reliable and standardized results.