Used in microbiology laboratories for the selective detection and differentiation of *Listeria* species, particularly *Listeria monocytogenes*, from other bacteria in food, environmental, and clinical samples. The medium contains chromogenic substrates that allow *Listeria* colonies to produce a distinct color—often blue or green—making identification rapid and easy without the need for further biochemical testing. It is widely applied in food safety testing to monitor for contamination in dairy, meat, and ready-to-eat products. The selective agents in the medium suppress the growth of non-*Listeria* organisms, improving accuracy. Its use supports compliance with food safety standards and helps prevent outbreaks of listeriosis.