Used primarily in microbiology for the cultivation and isolation of bacteria and fungi that require manganese as a growth supplement. The medium supports the growth of microorganisms involved in environmental testing, especially in soil and water analysis where manganese-dependent metabolic activities are studied. It is also applied in agricultural research to assess microbial populations that contribute to manganese cycling in the rhizosphere, aiding in plant nutrient uptake. In industrial settings, it helps in screening microbial strains capable of manganese oxidation or reduction, useful in bioremediation of metal-contaminated sites.