Beef Heart Infusion is widely used in microbiology as a nutrient-rich base for culture media. It provides essential amino acids, vitamins, and minerals that support the growth of fastidious microorganisms, especially bacteria requiring complex nutrients. It is commonly used in the preparation of media for cultivating pathogens such as Streptococcus and Leptospira. Due to its high nutritional content, it is also used in enrichment broths and in the production of vaccines and diagnostic reagents. Its ability to enhance microbial yield makes it valuable in clinical, veterinary, and research laboratories.