Used in microbiology laboratories for the detection and enumeration of tetracycline-resistant microorganisms. It helps assess microbial resistance patterns by inhibiting the growth of susceptible strains while allowing resistant organisms to grow. Commonly applied in environmental monitoring, food safety testing, and pharmaceutical quality control to evaluate contamination and resistance development. The agar provides a selective medium where only microbes capable of withstanding tetracycline can form colonies, enabling easy identification and quantification.