Used primarily in microbiology laboratories for the cultivation and detection of Clostridium species, particularly anaerobic spore-forming bacteria. Supports the growth of pathogenic strains like Clostridium perfringens, Clostridium difficile, and Clostridium botulinum. Commonly employed in food safety testing, clinical diagnostics, and environmental monitoring to identify contamination in samples such as feces, soil, and food products. Often used in conjunction with selective agents and anaerobic incubation to enhance specificity. Helps in studying spore germination, toxin production, and antibiotic susceptibility of Clostridium under controlled conditions.