Used primarily in microbiological testing to detect and cultivate iron-oxidizing bacteria in water systems. It supports the growth of bacteria that metabolize iron, making it essential for monitoring biofouling in pipelines, wells, and cooling systems. Commonly applied in environmental testing, water treatment plants use this medium to assess microbial activity that contributes to iron corrosion and sediment buildup. It helps identify bacterial presence in groundwater and industrial water systems, aiding in the prevention of infrastructure damage and water quality degradation. Also used in research to study microbial iron metabolism and bioremediation processes.