Used as a selective ionophore in chemical sensors and ion-selective electrodes, particularly for detecting alkali metal ions such as sodium (Na⁺). Its structure allows it to form stable complexes with cations of specific size and charge, enhancing selectivity in analytical devices. Also employed in colorimetric assays due to the presence of the dinitrophenylazo chromophore, which produces visible color changes upon ion binding, making it useful in optical sensors and educational demonstrations of molecular recognition. Additionally, it finds use in supramolecular chemistry for studying host-guest interactions in solution.