This chemical is primarily used in the field of bioconjugation and protein modification. It serves as a linker or spacer molecule in the synthesis of peptide and protein conjugates. The presence of the reactive pyrrole dione group allows it to form stable covalent bonds with thiol groups in proteins, making it valuable for labeling or functionalizing biomolecules. It is often employed in the development of antibody-drug conjugates (ADCs), where it helps connect therapeutic agents to antibodies for targeted drug delivery. Additionally, it is utilized in the preparation of biomaterials and hydrogels for applications in tissue engineering and drug delivery systems. Its stability and reactivity make it a versatile tool in biochemical research and pharmaceutical development.