Used as a crosslinking agent in bioconjugation processes, particularly for attaching biomolecules like proteins, peptides, or antibodies to surfaces or other molecules. Its amine-reactive NHS ester group enables efficient coupling with amine-containing compounds, while the thiol group allows attachment to thiol-reactive surfaces or moieties, making it valuable in creating stable conjugates for diagnostic assays, drug delivery systems, and research applications. It is also employed in modifying nanoparticles or polymers to enhance their functionality in biomedical and material science fields.