Bis(2,5-dioxopyrrolidin-1-yl) succinate is a homobifunctional crosslinker with two N-hydroxysuccinimide (NHS) ester groups, commonly used in bioconjugation to form stable amide bonds with primary amines on proteins, peptides, and other biomolecules. It facilitates the modification of proteins and peptides by creating covalent linkages to other molecules, such as antibodies or enzymes. This compound is essential in biochemical and immunological research for studying molecular interactions, developing diagnostic assays, and producing vaccines and therapeutics. Additionally, it supports the synthesis of biocompatible materials, including hydrogels for controlled drug delivery and functionalized polymers for medical devices.