This compound is widely used in peptide synthesis as a coupling agent, facilitating the formation of amide bonds between amino acids. It is particularly effective in activating carboxylic acids, making them more reactive towards amines. In biochemistry and molecular biology, it is employed for crosslinking proteins or nucleic acids, enabling the study of molecular interactions and structures. Additionally, it finds application in the modification of biomolecules, such as conjugating proteins with other molecules for diagnostic or therapeutic purposes. Its water-soluble nature makes it suitable for use in aqueous environments, enhancing its utility in biological research and pharmaceutical development.