N,N-Methylenebisacrylamide is widely used as a crosslinking agent in the production of polyacrylamide gels, which are essential in various biochemical and molecular biology applications. It plays a critical role in the formation of stable gel matrices for electrophoresis, particularly in techniques like SDS-PAGE, used for protein separation and analysis. Additionally, it is utilized in the synthesis of hydrogels for biomedical applications, including drug delivery systems and tissue engineering scaffolds, due to its ability to create a robust and biocompatible network. In industrial settings, it is employed in water treatment processes to enhance the efficiency of flocculants for purifying water by removing suspended particles.