Di(N-succinimidyl) pimelate is widely used as a crosslinking agent in biochemical and biotechnological applications. It is particularly effective in conjugating proteins, peptides, and other biomolecules by forming stable amide bonds. This compound is often employed in the preparation of antibody-enzyme conjugates, which are essential in immunoassays such as ELISA. Additionally, it is utilized in the study of protein-protein interactions, helping researchers understand the structure and function of complex biomolecular systems. Its ability to create covalent linkages between molecules makes it valuable in the development of biosensors and diagnostic tools. In material science, it is used to modify surfaces for immobilizing biomolecules, enhancing the functionality of various biomedical devices.