Disuccinimidyl glutarate (DSG) is widely used as a crosslinking agent in biochemistry and molecular biology. It enables the formation of stable covalent bonds between primary amine groups on proteins or other biomolecules, making it valuable for protein-protein conjugation, antibody labeling, and immobilization of enzymes on solid supports.

Due to its cell-membrane permeability, DSG is particularly useful for intracellular crosslinking studies, allowing researchers to capture transient protein-protein interactions within living cells. It is also employed in the development of diagnostic assays and therapeutic conjugates where precise linkage of biomolecules is required.

In addition, DSG is applied in proteomics research to stabilize protein complexes prior to analysis by techniques such as mass spectrometry or gel electrophoresis. Its spacer arm length provides sufficient flexibility for effective crosslinking without causing steric hindrance. The NHS ester groups react efficiently under mild conditions, and the resulting amide bonds are stable, ensuring durable conjugates for downstream applications.