Used as a crosslinking agent in bioconjugation, particularly for linking proteins, peptides, or other amine-containing molecules. Its NHS ester groups react efficiently with primary amines to form stable amide bonds, making it valuable in the preparation of labeled biomolecules and immobilization on surfaces. Commonly applied in the development of diagnostic assays, biosensors, and functionalized coatings where controlled spacing and biocompatibility are important. The spacer arm provides adequate length and flexibility, reducing steric hindrance in conjugated products. Also utilized in hydrogel formation and tissue engineering scaffolds due to its ability to link polymer chains under mild conditions.