Used as a bifunctional crosslinking agent in bioconjugation, particularly in the development of antibody-drug conjugates (ADCs) and peptide modifications. Its multiple carboxylic acid and amine-reactive groups allow for sequential coupling with biomolecules and payloads. The tert-butoxycarbonyl (Boc) protected amine enables controlled deprotection under mild acidic conditions, facilitating stepwise synthesis. Commonly employed in pharmaceutical research to construct stable, water-soluble linkers with defined spacers, improving the pharmacokinetics and targeting of therapeutic compounds. Also utilized in polymer chemistry to introduce branching points or functional handles in hydrogels and biocompatible materials.