Dimethyl suberimidate dihydrochloride is widely used as a crosslinking agent in biochemical and biophysical research. It is particularly effective in studying protein-protein interactions and protein structure due to its ability to form covalent bonds between amino groups in proteins. This chemical is often employed in the preparation of protein conjugates, which are essential for various immunological assays, including ELISA and immunohistochemistry. Additionally, it is utilized in the stabilization of protein complexes for structural analysis techniques such as X-ray crystallography and electron microscopy. Its application extends to the modification of enzymes and antibodies, enhancing their stability and functionality for therapeutic and diagnostic purposes.