Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is widely used as a crosslinking agent in biochemical and bioconjugation applications. It is particularly effective in linking biomolecules, such as proteins, peptides, and antibodies, due to its reactive N-hydroxysuccinimide (NHS) ester groups. These groups enable efficient coupling with primary amines, facilitating the formation of stable amide bonds. This compound is commonly employed in the preparation of immunoconjugates, enzyme immobilization, and the development of biosensors. Its ability to create strong and specific linkages makes it valuable in research and diagnostic tools, including ELISA assays and protein microarray technologies. Additionally, it is utilized in the synthesis of polymeric materials and drug delivery systems, where controlled crosslinking is essential for stability and functionality.