(2-hydroxy-5-nitrophenyl)boronic acid is primarily used in organic synthesis and chemical research due to its boronic acid functional group, which is highly reactive in forming covalent bonds with diols and other nucleophiles. This property makes it valuable in the development of Suzuki-Miyaura cross-coupling reactions, a widely used method for creating carbon-carbon bonds in the synthesis of pharmaceuticals, agrochemicals, and organic materials. Additionally, it serves as a key intermediate in the preparation of sensors and probes for detecting sugars, catechols, and other biologically relevant molecules, owing to its ability to form stable complexes with these compounds. Its nitro and hydroxyl groups further enhance its utility in designing fluorescent or colorimetric sensors for analytical applications.