Antalarmin hydrochloride is primarily used in research settings to study the effects of stress and anxiety. It acts as a corticotropin-releasing hormone (CRH) receptor antagonist, making it valuable for understanding the role of CRH in stress-related disorders. Researchers utilize it to explore potential therapeutic applications for conditions like anxiety, depression, and post-traumatic stress disorder (PTSD). Additionally, it is employed in animal studies to investigate behavioral responses to stress and to evaluate the efficacy of CRH antagonists in modulating stress pathways. Its application is largely confined to preclinical studies, providing insights into the neurobiological mechanisms of stress and potential drug development.