HDBTU is widely used as a coupling reagent in peptide synthesis. It facilitates the formation of peptide bonds between amino acids, making it essential in the production of peptides for pharmaceutical and biochemical research. Its efficiency and low racemization rate make it a preferred choice in solid-phase peptide synthesis. Additionally, HDBTU is employed in the synthesis of complex organic molecules, particularly in medicinal chemistry, where it aids in the creation of drug candidates and active pharmaceutical ingredients. Its stability and reactivity under mild conditions further enhance its utility in various chemical transformations.