Used as a coupling reagent in peptide synthesis, it facilitates the formation of amide bonds between amino acids. It is particularly effective in solid-phase peptide synthesis, enabling the efficient production of peptides with high purity and yield. The compound is also employed in the synthesis of complex organic molecules, where it helps in the activation of carboxylic acids for subsequent reactions. Its stability and reactivity make it a preferred choice in various chemical transformations, including the preparation of pharmaceuticals and bioactive compounds. Additionally, it is utilized in the modification of proteins and other biomolecules, aiding in the study of their structure and function.