This chemical is widely used as a coupling agent in peptide synthesis, facilitating the formation of amide bonds between amino acids. It is particularly effective in activating carboxylic acids, making them more reactive toward amines. In bioconjugation, it is employed to link biomolecules, such as proteins or nucleic acids, to other molecules or surfaces, which is essential in diagnostic assays and drug delivery systems. Additionally, it plays a role in the modification of polymers and the preparation of hydrogels, contributing to advancements in materials science and tissue engineering. Its applications also extend to organic synthesis, where it is used to create esters, thioesters, and other derivatives, enhancing the efficiency of chemical reactions.