Used extensively in peptide synthesis to facilitate the formation of amide bonds between amino acids. It is particularly effective in coupling carboxyl groups to amines, making it a crucial reagent in the production of peptides and proteins. Additionally, it finds application in the modification of polymers and surfaces, where it aids in the attachment of functional groups or biomolecules. In biochemistry, it is employed to crosslink proteins or nucleic acids, enabling the study of molecular interactions. Its role in activating carboxylic acids for further reactions also makes it valuable in organic synthesis, especially in the preparation of esters and amides.