1-Hydroxybenzotriazole hydrate is widely used as an additive in peptide synthesis to enhance coupling efficiency. It acts as a catalyst, reducing racemization and improving the yield of peptide bonds during the formation process. This compound is particularly valuable in solid-phase peptide synthesis, where it helps to activate carboxylic acids, making them more reactive with amines. Additionally, it is employed in organic synthesis for the preparation of amides, esters, and other derivatives, where it facilitates smoother and more efficient reactions. Its role in reducing side reactions and improving product purity makes it a crucial reagent in both research and industrial applications.