This chemical is primarily used as a fluorescent labeling reagent in biochemical and cellular studies. It reacts with amino groups in proteins, peptides, and other biomolecules, enabling their detection and analysis through fluorescence microscopy or spectroscopy. Its hydroxycoumarin moiety provides strong fluorescence, making it useful for tracking molecular interactions, studying protein dynamics, and visualizing cellular processes. Additionally, it is employed in the development of biosensors and assays to measure enzyme activity or monitor biochemical pathways. Its stability and sensitivity make it a valuable tool in research involving live-cell imaging and molecular diagnostics.