AMCA-X, N-succinimidyl ester is widely used as a fluorescent labeling reagent in biochemical and cellular research. Its primary application is in the conjugation of biomolecules, such as proteins, peptides, and antibodies, for fluorescence microscopy and flow cytometry. The compound’s succinimidyl ester group allows for efficient covalent attachment to primary amines, ensuring stable labeling. Its blue fluorescence emission makes it suitable for multiplexing with other fluorophores. Additionally, it is employed in studying protein interactions, cellular localization, and tracking molecular dynamics in live or fixed cells. Its water solubility and compatibility with biological systems enhance its utility in various experimental setups.