Widely used in bioconjugation, this compound serves as a fluorescent labeling reagent for biomolecules such as proteins, peptides, and antibodies. Its N-hydroxysuccinimide (NHS) ester group reacts efficiently with primary amines under mild conditions, forming stable amide bonds. The 7-hydroxy-4-methylcoumarin moiety provides strong fluorescence with high quantum yield, making it ideal for detection and imaging applications.

Commonly applied in fluorescence-based assays, it enables real-time tracking of cellular uptake, receptor binding, and enzyme activity. It is also used in the development of fluorescent probes and sensors due to its sensitivity and photostability. In drug delivery research, this reagent helps label nanocarriers and monitor their distribution in biological systems. Additionally, it supports high-throughput screening in diagnostic and pharmaceutical settings where fluorescent tags are essential.