Used as a fluorescent probe in biochemical assays due to its ability to emit light upon excitation, enabling detection of enzyme activity and molecular interactions. Commonly applied in studies involving hydrolases and transferases, where changes in fluorescence signal indicate catalytic action. Also utilized in the development of sensors for metal ions, particularly mercury and copper, owing to its selective response in the presence of these analytes. Its thiocarbonyl group enhances sensitivity and selectivity in recognition processes. Employed in research settings for real-time monitoring of cellular processes and in designing chemosensors for environmental monitoring.