Widely used as a fluorescent probe in biological imaging due to its strong emission in the visible range and good photostability. It is particularly effective in labeling cellular components and monitoring membrane dynamics because of its lipophilic nature, allowing easy penetration into lipid bilayers. Commonly employed in fluorescence microscopy and flow cytometry for real-time tracking of intracellular processes. Also utilized in the development of chemosensors for detecting metal ions and pH changes in environmental and physiological samples. Its high quantum yield and sensitivity to microenvironment changes make it valuable in studying protein interactions and conformational changes.