Used as a fluorescent probe in biochemical research, 3-Cyanoumbelliferone helps in studying enzyme activities and detecting specific biomolecules due to its strong fluorescence properties. It is also applied in organic synthesis as a building block for creating more complex compounds, particularly in the development of pharmaceuticals and agrochemicals. Additionally, it serves as a key intermediate in the production of dyes and optical brighteners. Its unique properties make it valuable in analytical chemistry for the detection and quantification of various substances. Furthermore, it is utilized in the development of luminescent materials for applications in electronic devices and sensors, thanks to its ability to emit light that changes with the chemical environment.