4-Ethoxycoumarin is widely used in the field of organic synthesis as a key intermediate for the preparation of various pharmaceuticals and biologically active compounds. Its structure serves as a building block for the development of anticoagulant agents, which are critical in the treatment of blood clotting disorders. Additionally, it is employed in the synthesis of fluorescent dyes and probes, which are essential tools in biochemical research for tracking and imaging biological processes. In the cosmetic industry, 4-Ethoxycoumarin is utilized in the formulation of fragrances due to its pleasant aromatic properties. It also finds application in the study of enzyme kinetics, particularly as a substrate for cytochrome P450 enzymes, helping researchers understand drug metabolism and interactions.