Basic cupric sulfate is primarily used as a fungicide in agriculture, especially in vineyards and orchards to control fungal diseases like downy and powdery mildew. It is commonly incorporated into Bordeaux mixture formulations, where it reacts with lime to form a protective coating that inhibits spore germination and fungal growth.

It also serves as an intermediate in the synthesis of other copper-based compounds for industrial applications, including pigments for paints, ceramics, and inks due to its stability and blue-green coloration.

In chemical processes, it acts as a heterogeneous catalyst in certain organic reactions and as an additive in analytical chemistry.

Additionally, it is used in educational and laboratory settings for demonstrations of precipitation, insoluble salts, and qualitative analysis of copper ions.