Copper(II) gluconate is widely used as a dietary supplement due to its high bioavailability and low toxicity. It serves as a source of copper in nutritional formulations, helping prevent and treat copper deficiency in humans and animals. It is commonly found in multivitamin tablets, mineral supplements, and fortified foods.

In medicine, it is used in lozenges and oral solutions aimed at supporting immune function and connective tissue formation, as copper plays a vital role in enzymatic reactions involving iron metabolism and red blood cell synthesis.

It is also employed in electroplating baths, where it acts as a copper source for depositing uniform and adherent copper coatings on various substrates, especially in mild pH conditions where traditional copper salts might be too corrosive.

In research and biotechnology, copper(II) gluconate is used as a catalyst or reagent in organic synthesis and in the preparation of copper-doped nanomaterials. Its mild reactivity and water solubility make it suitable for use in biochemical assays and enzyme studies.

Additionally, it finds application in agriculture as a micronutrient in animal feed and in some plant nutrient solutions to correct copper deficiency in soils.