Semixylenol orange is widely used as a metallochromic indicator in complexometric titrations, particularly for the determination of metal ions such as calcium, magnesium, and zinc. It forms colored complexes with these metal ions, allowing for precise endpoint detection in titration processes. Additionally, it is employed in biochemical and environmental research to study metal ion interactions and their presence in various samples. Its sensitivity to metal ions makes it valuable in analytical chemistry for quantifying trace metal concentrations in water, soil, and biological materials.