Used extensively in biochemistry and biotechnology for ion exchange chromatography, particularly for the purification of proteins, peptides, and nucleic acids. Its carboxymethyl (CM) groups provide strong cation exchange properties, allowing binding of positively charged molecules at specific pH ranges. The matrix, derived from cross-linked agarose (Sepharose CL-6B), offers high mechanical stability and low nonspecific binding, making it suitable for both laboratory-scale and preparative separations. Commonly applied in the purification of antibodies, enzymes, and viral particles due to its high capacity and resolution. Can be used in gravity columns, FPLC, or HPLC systems under moderate pressure. Reusable after proper cleaning and regeneration, making it cost-effective for repeated purification processes.