Used as a redox indicator in analytical chemistry, this compound undergoes a distinct color change upon reduction or oxidation, making it valuable in titrations and redox potential measurements. It is particularly effective in biological and biochemical applications, such as assessing microbial activity or enzyme function, where electron transfer processes are monitored. Its sharp color transition allows for visual or spectrophotometric detection, enhancing accuracy in determining endpoint in redox reactions. Also employed in diagnostic tests and dye-based assays where reliable electron acceptor behavior is required.