Used as a chromogenic substrate in biochemical assays, particularly in enzyme detection. It is commonly employed in histochemistry and microbiology for the visualization of enzymatic activity, such as β-galactosidase and esterase. Upon enzymatic hydrolysis, it releases a blue-colored product, enabling easy detection of enzyme-positive colonies or cells under light microscopy. Its diacetate groups enhance cell membrane permeability, making it effective in live-cell imaging and staining applications. Widely used in agar-based media for rapid identification of microbial strains in clinical and environmental samples.