Stilbazo is primarily utilized as a metallochromic indicator in analytical chemistry, particularly in complexometric titrations. It is effective in detecting metal ions, such as calcium and magnesium, by producing a distinct color change when it binds to these ions. This property makes it valuable in water hardness testing and the analysis of metal concentrations in various samples. Additionally, Stilbazo is employed in spectrophotometric methods for quantitative analysis due to its ability to form stable complexes with specific metals, enabling precise measurements in laboratory settings.