3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate
98%
Reagent
Code: #126770
Alias
TMB HCL ; 3,3',5,5'-tetramethylbenzidine dihydrochloride dihydrate
CAS Number
64285-73-0
science Other reagents with same CAS 64285-73-0
blur_circular Chemical Specifications
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Molecular Information
Weight
313.27 g/mol
Formula
C₁₆H₂₂Cl₂N₂
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Registry Numbers
EC Number
264-769-6
MDL Number
MFCD00012961
thermostat
Physical Properties
Melting Point
≥300 °C
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Storage & Handling
Storage
2~8°C
description Product Description
Used extensively as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) for detecting peroxidase activity. It produces a blue color upon oxidation, which turns yellow when acid is added, allowing for quantitative analysis. Commonly applied in clinical diagnostics for detecting antibodies, antigens, and hormones. Also utilized in immunohistochemistry for visualizing tissue samples and in research laboratories for studying enzyme kinetics. Its sensitivity and stability make it a preferred choice in various biochemical and medical applications.
format_list_bulleted Product Specification
| Test Parameter | Specification |
|---|---|
| Purity (HPLC) (%) | 98 |
| Purity (Titration With AgNO3) (%) | 97.5-102.5 |
| Water (Karl Fischer, %) | 10-12 |
| Appearance | White or light yellow powder |
| Infrared Spectrum | Conforms to Structure |
| Solubility in Acetic Acid:Water (1:2) | Clear to slightly hazy, yellow to green at 20 mg/mL |
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