Triphenylformazan Nitrogen is widely used in analytical chemistry as a reagent for the detection and quantification of metal ions, particularly copper and iron. Its ability to form colored complexes with these metals makes it valuable in spectrophotometric analysis, where it aids in determining metal concentrations in various samples, including environmental and biological specimens.

In biochemistry, it serves as a redox indicator, helping to monitor oxidation-reduction reactions in enzymatic studies. Its sensitivity to changes in oxidation states allows researchers to track electron transfer processes, providing insights into metabolic pathways and enzyme mechanisms.

Additionally, Triphenylformazan Nitrogen is employed in material science for the development of dyes and pigments. Its stable and vibrant color properties make it suitable for use in coatings, inks, and other colorant applications, where durability and consistency are essential.

In research, it is utilized as a staining agent in histology and microscopy to enhance the visibility of cellular structures. Its ability to bind selectively to certain tissues or components aids in the study of cell morphology and function.